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. 2020 Feb 15;8(2):260. doi: 10.3390/microorganisms8020260

Table 1.

Sequences of primers used for gene amplification.

Virulence Genes (Product Length) Primers (5′-3′)
exoS (235 bp) FWD: AGAGCGAGGTCAGCAGAGTA
REV: GCGGACATACCTTGGTCGAT
exoT (219 bp) FWD: GCATGCGGTAATGGACAAGG
REV: GACCGATTCAGGTGCTGGTA
exoU (134 bp) FWD: CGGTACGTGCTGTATCCCTC
REV: CGTGTAGCGCGATCTGTAGT
exoY (289 bp) FWD: GCTTCTCGGTGAAGGGGAAA
REV: CGAACTCATAGCGTTTGCCG
lasB (202 bp) FWD: ATCGACGTGTCCAAACTCCC
REV: CCTTGACTTCGGTGATGGCT
aprA (176 bp) FWD: CTACAGCGCCAACGTCAATC
REV: AGCTCATCACCGAATAGGCG
ladS (181 bp) FWD: CCCTGATGGTCCTCGGCTAC
REV: GTTCCTGGTTCAGCGCTTCC
pscL FWD: AAAAAAGAATTCGGAGGGCGATGAATGCTTCCATTTGTT
REV: AAAAAAAAGCTTTCAACCGGCGTCCCCTTCCTCCT
pscU FWD: AAAAAATCTAGAGGAGGAGACGCCATGAGCGCCGAGAAGA
REV: AAAAAAAAGCTTGATAGCGATCAGGGCGTATCCGTCTGCT
prpL FWD: ATCGTATTTCGCCGACTCCC
REV: TGAAGACCATCTTCGCCACC