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IAA in vitro production by the wild-type Psn23 (WT) and its mutants ΔmatE, pT3-matE, ΔiaaM, and ΔiaaL. After 24 h (A) and 48 h (B) of in vitro growth on MM supplemented with Trp, free IAA and IAA-Lys were quantified in the bacterial supernatants by HPLC–FLD. Data are averages of triplicates ± standard deviation (SD). Different letters indicate significant differences among means of mutants at p < 0.05, according to Tukey’s test.
