Figure 4.

Effect of planktonic and biofilm-associated Salmonella on neutrophil ROS production. JSG4383 used for WT S. Typhi. (A,B) CFU enumeration of total (intracellular and extracellular) planktonic and biofilm aggregate survival for each WT and all mutants 80 minutes post-infection. No significant differences in survival were identified by two-way ANOVA with Sidak’s method for multiple comparisons (n = 3, enumerated in triplicate). Error bars indicate SD. (C,D) Reactive oxygen species (ROS) production from neutrophils challenged with planktonic or biofilm aggregates of WT and all EPS mutants. Daily experiments were normalized by a phorbol 12-myristate 13-acetate (PMA)-stimulated control and the data shown are representative of three independent experiments, each with similar trends.