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. 2020 Feb 13;8(2):253. doi: 10.3390/microorganisms8020253

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sensitivity and tolerance to oxidative species. JSG4383 used for WT S. Typhi. (A,B) Growth of WT planktonic Salmonella in the presence of H₂O₂ demonstrating a minimum inhibitory concentration (MIC) of 2.5 mM for both serovars. (C,D) Growth of WT planktonic Salmonella in the presence of ClO⁻ demonstrating a MIC of 500 µg/ml for both serovars. (E–I) CFU enumeration of biofilm aggregates challenged with H₂O₂ supplied at 1× (2.5 mM), 10× (25 mM), 25× (62.5 mM), or 50× (125 mM) the WT planktonic MIC or ClO⁻ supplied at 1× (0.50 mg/mL), 10× (5 mg/mL), 25× (12.5 mg/mL), or 50× (25 mg/mL) the WT planktonic MIC. Significant reductions in tolerance were identified by comparing control (0×) CFUs with the CFUs of each concentration tested by two-way ANOVA with Tukey’s multiple correction method (**, p < 0.01; ***, p < 0.0005; ****, p < 0.0001). All experiments were conducted in triplicate and the data are the averages of three independent experiments. Error bars indicate SD.

Sensitivity and tolerance to oxidative species. JSG4383 used for WT S. Typhi. (A,B) Growth of WT planktonic Salmonella in the presence of H₂O₂ demonstrating a minimum inhibitory concentration (MIC) of 2.5 mM for both serovars. (C,D) Growth of WT planktonic Salmonella in the presence of ClO⁻ demonstrating a MIC of 500 µg/ml for both serovars. (E–I) CFU enumeration of biofilm aggregates challenged with H₂O₂ supplied at 1× (2.5 mM), 10× (25 mM), 25× (62.5 mM), or 50× (125 mM) the WT planktonic MIC or ClO⁻ supplied at 1× (0.50 mg/mL), 10× (5 mg/mL), 25× (12.5 mg/mL), or 50× (25 mg/mL) the WT planktonic MIC. Significant reductions in tolerance were identified by comparing control (0×) CFUs with the CFUs of each concentration tested by two-way ANOVA with Tukey’s multiple correction method (**, p < 0.01; ***, p < 0.0005; ****, p < 0.0001). All experiments were conducted in triplicate and the data are the averages of three independent experiments. Error bars indicate SD.