Table 4.
The three most common AMR gene PCR patterns z for M. haemolytica (MH), P. multocida (PM) and H. somni (HS) isolated from lung tissue of bovine respiratory disease mortalities. ‘+’ = present, ‘-’ = absent; ICE core gene fragmentsy; AMR gene fragments.
| Strain | hyp | int | rlx | mco | tnp | aph(3′) Ia | aph(6)Id | aph(3″)Ib | sul2 | floR | erm (42) | ant(2″)Ia | aadA25 | bla OXA-2 | msr (E) | mph (E) | tet (R) | tet (H) | Total Genes | # Isolates with Same Pattern (% of Total Isolates) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MH | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | 16 | 44/111 (39.6) |
| MH | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 1 | 23/111 (20.7) |
| MH | - | - | + | + | + | + | + | + | + | - | - | - | - | - | - | - | + | - | 8 | 14/111 (12.6) |
| PM | + | + | + | + | + | + | + | + | + | - | - | - | - | - | - | - | + | + | 11 | 22/47 (46.8) |
| PM | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0 | 10/47(21.3) |
| PM | + | + | + | + | + | - | - | - | - | - | - | - | - | - | - | - | + | + | 7 | 9/47(19.1) |
| HS | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0 | 11/42(26.2) |
| HS | + | + | + | + | - | - | - | - | + | + | - | - | - | - | + | + | + | + | 10 | 7/42 (16.7) |
| HS | + | + | + | + | - | + | + | + | + | + | - | - | - | - | + | + | + | + | 13 | 3/42 (7.1) |
z Additional information regarding PCR primers shown in Table 1. y ICE core gene fragments: hyp, hypothetical protein; int, integrase; rlx, relaxase; mco, multi-copper oxidase; tnp, transposase.