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. 2020 Feb 21;8(2):299. doi: 10.3390/microorganisms8020299

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Screening of carbohydrate targets for the peptides using a solution carbohydrate array. The array contained different terminal sequences of N-glycans, O-glycans, and glycosphingolipids of microbial cells and mammalian tissues. Briefly, donor beads (500 ng/well) and biotin-PAA-sugars were mixed with FITC-P-113 and FITC-P-113Tri. Subsequently, a mixture of acceptor beads, mouse anti-FITC antibody, and rabbit anti-mouse IgG antibody was added. The binding signals were analyzed with a PerkinElmer Envision instrument using AlphaScreen^TM. The top 40 glycans with peptide binding are shown. Le^d (H type 1): Fucα1-2Galβ1-3GlcNAcβ, Le^b: Fucα1-2Galβ1-3(Fucα1-4)GlcNAcβ, Le^y: Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ, 3’sulfate Le^a: 3-HSO₃-Galβ1-3(Fucα1-4)GlcNAcβ, 3’sulfate Le^x: 3-HSO₃-Galβ1-4(Fucα1-3)GlcNAcβ, 6GlcNAc-HSO₃-Sia Le^x: Neu5Acα2-3Galβ1-4(Fucα1-3)(6-HSO₃)GlcNAcβ, 6Gal-HSO₃-Sia Le^x: Neu5Acα2-3(6-HSO₃)Galβ1-4(Fucα1-3)GlcNAcβ. Gal: galactose; GalNAc: N-acetylgalactosamine; Glc: glucose; GlcNAc: N-acetylglucosamine; NeuAc: N-acetylneuraminicacid; NeuGc: N-glycolylneuraminicacid. Green stars: glycans presented in human cells; Blue stars: glycans presented in microbial cells.