Fig. 1.

Aqp4 expression in retinae of β1-syn KO mice. a Immunoblot from total protein lysates of WT and β1-syn KO retina samples. β1-syn KO mice lack an immunoreactive protein band at ~ 59 kDa corresponding to β1-syn, confirming the genotype. b qPCR analysis of total RNA extracted from eye using primers specific for Aqp4. No statistically significant difference in total Aqp4 gene expression was observed between the two genotypes (n = 6 for WT and n = 7 for β1-syn KO). Statistics: Mann-Whitney U-test. Tbp was used as the normalization gene. Data shown as mean ± SEM. c Immunoblot showing AQP4 expression in total protein lysates from WT and β1-syn KO retinae. Statistical analysis of AQP4 expression in WT and β1-syn KO showed no difference in AQP4 protein expression between the two genotypes (n = 5 for each group). Densitometric values are expressed as percentage of average WT values ± SD. Statistics: independent samples t-test. GAPDH was used as the loading control