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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2019 Dec 26;147:242–251. doi: 10.1016/j.freeradbiomed.2019.12.023

Fig. 2.

Fig. 2

J20 mice exposed to chronic nPM had increased 4-HNE in cerebral cortex, but not cerebellum. a, c 4-HNE immunohistochemistry: a cerebral cortex. c cerebellum. b, d Quantification of 4-HNE immunostaining. b cerebral cortex: 2-fold increase of 4-HNE (p<0.001) from nPM exposure; b 4-HNE in cortical layers 2-4: d cerebellum 4-HNE, no change. e 4-HNE immunoblots of lipid raft enriched fractions, Ponceau red staining as a loading control for protein. f Quantified 4-HNE immunoblots of panel e: nPM exposure increased 4-HNE in lipid rafts from cortex (p < 0.05), but not cerebellum. ctrl= 7, nPM= 8 mice. Scale bar: 100 μm; mean ± SEM. * p<0.05, *** p<0.005, ****p<0.001.