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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2019 Dec 26;147:242–251. doi: 10.1016/j.freeradbiomed.2019.12.023

Fig. 4.

Fig. 4

In vitro N2a-APP/swe cell lipid raft responses to nPM. a Western blots for APP-FL (full length), PS1, BACE1 and flotillin-1 in lipid raft b Total APP was decreased by nPM exposure with dose-dependence. c PS1 had a trend for increase at higher nPM (p=0.06). d BACE1 and e flotillin-1 did not respond to nPM. f Lipid raft fractions stained for cholera toxin B (Ctx-B), nuclear staining (DAPI, middle panels) and merged images; scale bar, 15 μm. Control (ctrl, globular, upper panels) and nPM exposed cells (puncta, lower panels). g nPM at all doses increased Ctx-B puncta-like staining of lipid rafts, with statistical significance at 5 μg/ml nPM. n=3 independent experiments, 3 samples each. Mean ± SEM * p<0.05, ** p<0.01, *** p<0.005.