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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: Cell Rep. 2019 Jan 15;26(3):802–813.e4. doi: 10.1016/j.celrep.2018.12.088

Figure 4. Photostability Analysis of GEVIs.

Figure 4.

(A) One-photon photobleaching kinetics of GEVIs. Cultured neurons expressing ArcLight Q239 (n = 6 cells), ArcLight-MT (n = 6 cells), ASAP1 (n = 6 cells), ASAP2f (n = 7 cells), ASAP2s (n = 9 cells), Ace2N-4AA-mNeon (n = 7 cells), QuasAr2 (n = 11 cells), and Archer1 (n = 8 cells) are continuously illuminated with a mercury arc lamp.

(B) Time constants by 1-photon illumination (means ± SEMs).

(C) Two-photon photobleaching kinetics of GEVIs. Cultured neurons expressing ArcLight Q239 (n = 8 cells), ArcLight-MT (n = 7 cells), ASAP1 (n = 6 cells), ASAP2f (n = 9 cells), ASAP2s (n = 11 cells), and Ace2N-4AA-mNeon (n = 8 cells) are continuously scanned at 30 Hz.

(D) Time constants by 2-photon illumination (means ± SEMs).

See also Figure S2