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. 2020 Feb 5;10(2):266. doi: 10.3390/nano10020266

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The presence of iron and nanoparticles in J774A.1 cell line incubated 24 h with or without FERINJECT^® or Fe₂O₃-NP Sigma. Panel (A): Perls staining. Blue staining: complex KFe [Fe(CN)6] named Prussian blue. Red staining: Safranin cytosolic staining; Panel (B): TEM microscopy. Scale bar, top line = 2 µm; below line = 0.5 µm (Control) or 0.2 µm (FERINJECT^® and Fe₂O₃-NP Sigma). Panel (C): Top line: HAADF (High-Angle Annular Dark Field microscopy) with, in white high-density zone. Below line: EDX (Energy Dispersive X-ray Analysis) with, in red iron elements. N = Nucleus; M = Microvillosity; R = Reticulum; MVB = Multivesicular bodies; arrow = vesicle with iron particles. Panel (D): Quantitative assessment of the iron engulfed by macrophages using the bathophenanthroline method.

The presence of iron and nanoparticles in J774A.1 cell line incubated 24 h with or without FERINJECT^® or Fe₂O₃-NP Sigma. Panel (A): Perls staining. Blue staining: complex KFe [Fe(CN)6] named Prussian blue. Red staining: Safranin cytosolic staining; Panel (B): TEM microscopy. Scale bar, top line = 2 µm; below line = 0.5 µm (Control) or 0.2 µm (FERINJECT^® and Fe₂O₃-NP Sigma). Panel (C): Top line: HAADF (High-Angle Annular Dark Field microscopy) with, in white high-density zone. Below line: EDX (Energy Dispersive X-ray Analysis) with, in red iron elements. N = Nucleus; M = Microvillosity; R = Reticulum; MVB = Multivesicular bodies; arrow = vesicle with iron particles. Panel (D): Quantitative assessment of the iron engulfed by macrophages using the bathophenanthroline method.