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. 2020 Mar 11;13:805–813. doi: 10.2147/IDR.S239906

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© 2020 Tang et al.

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Talaromyces marneffei yeast cells induced the activation of DCs and the secretion of IL-6, IL-10 and TGF-β. DCs were cultured with or without yeast cells of TM (DCs: yeasts=1: 5) for 24 h, and the percentages of DCs expressing CD80 and CD86 on the surface were detected by flow cytometry. (A) Representative histograms of CD80 (left panel) and CD86 (right panel) are shown for un-stimulated and yeast-stimulated DCs. Comparisons of the percentages of (B) CD80 and (C) CD86 on DCs. In addition, the concentrations of (D) IL-6, (E) IL-10, and (F) TGF-β in the culture supernatant were detected by ELISA. Data are expressed as mean± SD (n=10). *P < 0.05, ***P < 0.001.

Abbreviations: DC, dendritic cell; TM, Talaromyces marneffei; CD, cluster differentiation; IL-6, interleukin 6; IL-10, interleukin 10; TGF-β, transforming growth factor β; SD, standard deviation.