FIGURE 5.

Relationship between DMS reactivity and abundance and its modulation by salt stress. (A) Transcript mean reactivity in shoots is positively correlated with transcript abundance under control conditions. A similar direct relationship was observed for the shoot transcriptome under NaCl stress, as well as for the root transcriptome under both conditions (Supplemental Fig. S10, all tests in Supplemental Data S3: Tables S8A,B). (B) Transcripts within the top and bottom 5% most extreme salt stress-induced reactivity increases and decreases [Mean log₂(TPM) increases 5.77, decreases 3.73, t.test P = 2.2 × 10⁻¹⁶]. (C) Transcripts within the top and bottom 5% most extreme salt stress-induced abundance changes [Mean log₂(TPM) of decreases 5.89, increases 3.04, t.test P = 2.2 × 10⁻¹⁶]. Transcripts with high initial abundance in control conditions tend to increase in reactivity and decrease in abundance, while transcripts with initial low abundance tend to decrease in reactivity and increase in abundance (Supplemental Data S3: Table S8C). Similar patterns are observed for the root transcriptome (Supplemental Fig. S11; Supplemental Data S3: S8C). All data shown are under the initial, unstressed conditions, that is, reactivity increases are those transcripts that increase in reactivity after salt stress.