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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: Nat Protoc. 2018 Oct;13(10):2121–2148. doi: 10.1038/s41596-018-0016-7

Fig. 3 |. Bifunctional chelating agents and corresponding conjugated antibodies.

Fig. 3 |

a,b, Two types of bifunctional chelating agents (BFCAs) are complexed with tagging metal cations: monomeric DOTA (a) and polymeric DTPA (b). The selected antibody (Ab) is coupled with BFCAs via a biorthogonal reaction of maleimide moieties and sulfhydryl groups of cysteine residues in the hinge region between two heavy chains. c,d, Antibody conjugated with monomeric BFCAs (mBFCAs) (c) and antibody conjugated with polymeric BFCAs (pBFCAs) (d).