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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: Nat Protoc. 2018 Oct;13(10):2121–2148. doi: 10.1038/s41596-018-0016-7

Fig. 7 |. Barcoding live Jurkat cells using Pd-tagged CD45 antibodies.

Fig. 7 |

Live Jurkat cells were barcoded using three strategies for combining CD45 antibodies tagged with six palladium isotopes. The top section of each panel shows the CyTOF measurements as ‘rain dots’. The bottom sections show the biaxial contour plots of the indicated isotopes. a, 6-choose-1 barcoding strategy: Jurkat cells were stained with each of the six Pd-tagged CD45 antibodies. b, 6-choose-2 barcoding strategy: Jurkat cells were stained with 106Pd- and 108Pd-tagged CD45 antibodies, 108Pd- and 110Pd-tagged CD45 antibodies, or 106Pd- and 110Pd-tagged CD45 antibodies. c, 6-choose-3 barcoding strategy: Jurkat cells were stained with 102Pd-, 104Pd-, and 105Pd-tagged CD45 antibodies.