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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: Nat Protoc. 2018 Oct;13(10):2121–2148. doi: 10.1038/s41596-018-0016-7

Fig. 8 |. Titrations of various 209Bi-tagged antibodies.

Fig. 8 |

a, Titration histograms of 209Bi-tagged MHC class II antibody with mouse PBMCs and titration curves in B cells and T cells. b–e, Titration histograms at the optimal concentrations. b, 209Bi-tagged p-STAT3 antibody of 1,000 ng/mL with CD33+CD14+ monocytes in human PBMCs with stimulation of 100 ng/mL IL-10 as positive control and without stimulation as negative control. c, 209Bi-tagged p-histone H3 antibody of 2,000 ng/mL with mesodermal progenitors derived from human embryonic stem cells on day 6 of differentiation as positive control and cardiomyocytes derived from human embryonic stem cells as negative control. d, 209Bi-tagged IFN-γ antibody of 1,000 ng/mL with natural killer cells with stimulation of 50 ng/mL IL-12 and IL-18 as positive control and without stimulation as negative control. e, 209Bi-tagged anti-PE antibody of 1,000 ng/mL with PE-conjugated CD3-antibody-stained T cells and B cells in human PBMCs as positive and negative controls, respectively. cps, counts per second.