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. 2020 Mar 16;15(3):e0230381. doi: 10.1371/journal.pone.0230381

Fig 3. Immunization and challenge schedule, IgG titers and cytokine levels.

Fig 3

(A) Three weeks after DNA intramuscular injection, protein boosted immunization was carried out. Mice were sacrificed for immune analysis at 3rd, 6th and 9th week post-immunization. In addition, infections were performed, and mice were euthanized for immune analysis at 4th 8th and 12th week post-infection. (B, C) Post-immunization or post-infection IgG, IgG1 and IgG2a antibody titers were measured by ELISA, and the values of IgG1/IgG2a were calculated as well. (D) Post-infection cytokines, IL-4 and TNF-α, were determined by ELISA. The data were presented as mean ± SD. Statistical analyses were performed by IBM SPSS Statistics 22 with one-way analysis of variance, and the significant difference was designed as asterisks (*P<0.05, **P<0.01,***P<0.001).