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. 2020 Mar 16;15(3):e0230138. doi: 10.1371/journal.pone.0230138

Fig 1. Construction of dscCfaE-CTA2/CTB chimera.

Fig 1

(A) Map of 0809C304 plasmid. The dscCfaE, CTA2, and CTB genes are shown as red arrows, with the LTIIb-B gene signal peptide sequence shown as red triangles. The arabinose-inducible promoter (pBAD) and the araC promoter (pC) are shown as open red triangles Vector encoded araC (pBAD repressor) and cat (chloramphenicol resistance marker) are shown as green arrows. (B) Amino acid sequences for the dscCfaE-CTA2 and CTB genes. The LTIIb-B gene signal peptide sequence, which directs periplasmic export, is shown in italics, the amino acid sequence for mature dscCfaE is highlighted in bold, and the amino acid sequence for CTA2 is underlined. (C) Model of assembled dscCfaE-CTA2/CTB chimera. The dscCfaE-CTA2 fusion, which is noncovalently tethered to CTB (brown), is shown in pink (dscCfaE) and gray (CTA2). Model produced in UCSF Chimera software using crystal structures of dscCfaE (DOI: 10.2210/pdb2HB0/pdb) and cholera holotoxin (DOI: 10.2210/pdb1S5E/pdb).