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. 2020 Mar 16;15(3):e0230138. doi: 10.1371/journal.pone.0230138

Fig 3. Binding functionalities of the dscCfaE-CTA2/CTB chimera.

Fig 3

(A) Binding of chimera protein to solid-phase GM1 by ELISA. GM1-coated plates were incubated with chimera, dscCfaE, or CTB. Rabbit anti-dscCfaE and goat anti-rabbit IgG-HRP antibodies were used as the primary and secondary antibody, respectively. The bars represent mean absorbance at OD 450nm (+SEM of 4 replicate wells from one representative experiment). (B) In vitro adherence of chimera to bovine erythrocytes, measured by visual hemagglutination. Polystyrene beads were coated with chimera (1), dscCfaE (2) or CTB (3) and incubated with bovine erythrocytes.