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. 2020 Mar 16;15(3):e0230138. doi: 10.1371/journal.pone.0230138

Fig 5. Dose-dependent immune response elicited by the dscCfaE-CTA2/CTB chimera.

Fig 5

Serum antibody responses on day 42 in BALB/c mice vaccinated IN with 65 μg, 21 μg, 7 μg, or 2 μg, dscCfaE-CTA2/CTB (black bars), 13 μg CTB (stippled bars), 25 μg CfaE (striped bars), or PBS (white bars). (A) Anti-CfaE IgG, (B) anti-CfaE IgA, (C) anti-CTB IgG and (D) anti-CTB IgA end point ELISA titers are shown as mean log10 titers ± SD. The horizontal lines (1.7) denote the lowest dilution tested. (E) Functional antibody titers are shown, as measured by the HAI assay using CFA/I-ETEC (H10407) and red blood cells. Data are represented as the median and interquartile ranges of data from each group and the horizontal dotted line (1:32) denotes the lowest dilution of serum tested. For A-E, statistical significance was determined by using one-way ANOVA and Tukey’s multiple comparisons test. Asterisks denote a significant difference between responses in the indicated groups (*P<0.05; **P<0.01; ***P<0.001; ns—not significant).