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. 2020 Mar 16;15(3):e0230138. doi: 10.1371/journal.pone.0230138

Fig 6. Orogastric immunization with dscCfaE-CTA2/CTB chimera (Chim).

Fig 6

Serum antibody responses on day 42 in BALB/c mice vaccinated orogastrically with 162 μg dscCfaE-CTA2/CTB (black bars), CfaE + CTB admixture containing 62 μg dscCfaE and 91 μg CTB (grey bars), 62 μg CfaE alone (striped bars), or PBS (white bar). The admixture and CfaE groups were molar matched to the approximate amounts present in 162 μg chimera. (A) Anti-CfaE IgG, (B) anti-CfaE IgA, (C) anti-CTB IgG and (D) anti-CTB IgA end point ELISA titers are shown as mean log10 titers ± SD. The horizontal lines (1.7) denote the lowest dilution tested. (E) Functional antibody titers are shown, as measured by the HAI assay using CFA/I-ETEC (H10407) and red blood cells. Data are represented as the median and interquartile ranges of data from each group and the horizontal dotted line (1:32) denotes the lowest dilution of serum tested. For A-E, statistical significance was determined by using one-way ANOVA and Tukey’s multiple comparisons test. Asterisks denote a significant difference between responses in the indicated groups (**P<0.01; ***P<0.001).