Table 1. Wavelength shifts $(\Delta \lambda )$ achieved for the different immunoassays during incubation.

Curve	Antigen	Solution	${\Delta \lambda }_i$	${\Delta \lambda }_f$	$S_{\Delta \lambda }$
A	Yes	PBS	0	3.05	3.05
B	No	PS	0.49	4.01	3.52
C	No	HS	0.50	4.01	3.51
D	Yes	PS	0.75	4.78	4.03
E	Yes	HS	1.06	6.01	4.95