Fig 2. Validation of RNA-seq by RT-qPCR.

Validation was performed by repeating the experimental setup for RNA-seq with inclusion of a tcs07 mutant and performing RT-qPCR on the extracted RNA with primer sets for selected upregulated genes and hk07, nanA and bgaA. mRNA levels were normalized to gyrA and mRNA fold-changes are relative to the wild-type. Performed in biological duplicates with standard deviation as error bars.