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. Author manuscript; available in PMC: 2020 Mar 16.
Published in final edited form as: J Am Chem Soc. 2016 Sep 30;138(40):13353–13360. doi: 10.1021/jacs.6b07890

Figure 6.

Figure 6.

Fluorophore release in HEK293T cells through treatment with exogenous H2O2. Cells were treated with 33 (10 μM) in DPBS buffer for 45 min at 37 °C, followed by replacement with fresh DPBS containing (A) vehicle or (B) H2O2 (100 μM). After 30 min incubation, cellular fluorescence was imaged on a Zeiss Axio Observer Z1 microscope using a 20× objective and GFP (Set 38 HE) filter (ex = 470 nm, em = 525 nm). (C) Bright-field image of cells in panel B stained with Hoechst 33258 (1 μM) and imaged using a DAPI (Set 68) filter (ex = 377 nm, em = 464 nm). (D) Mean fluorescence intensities were calculated from individual ROIs (n = 3) and set relative to the mean fluorescence intensity prior to treatments (F/Fi). Error bars denote standard deviations.