Figure 32.

Fusion of PFV. (a) Bright field image of a cell overlaid with the trajectory of a dual-color PFV virus during fusion at or near the plasma membrane. The dual-color signal is shown in yellow and the single-labeled capsid in green. (b) Tracking image correlation (TrIC) analysis of the fusion event observed in panel (a). (upper panel) The fluorescence intensity of the Gag-eGFP signal (green) and mCherry-Env (red) signal, (second panel) instantaneous velocity, (third panel) cross-correlation amplitude (blue) and randomized cross-correlation signal (black), and (lower panel) relative distance between the Gag-eGFP and mCherry-Env signals plotted as a function of time. (c) 3D relative trajectory of the Env-labeled envelop with respect to the Gag-capsid showing movement in the order of hundreds of nanometers between the two labels before the completion of the fusion process. Adapted with permission from ref (467). Copyright 2013 Elsevier.