Figure 7.
Generation of recombinant influenza viruses carrying a GFP reporter. (a) Schematic representation of the NS segment of WT PR8 virus and NS1-GFP virus. (b) A549 cells were infected with recombinant PR8 virus carrying NS1-GFP. At 10 h postinfection, cells were fixed and stained for NP. NP staining is shown in red and NS1-GFP is shown in green. (c) Fluorescent micrographs of NS1-GFP virus plaques taken at 20× magnification. Adapted with permission from ref (217). Copyright 2010 National Academy of Sciences, U.S.A.