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. 2019 Dec 10;14(1):568–584. doi: 10.1021/acsnano.9b06905

Figure 3.

Figure 3

Reaction kinetics for the tetrazine ligation of PeptoBrushes. (A) Fluorogenic “turn-on” Tz 14 and 15 and water-soluble TCO derivative 16 used for kinetic investigations by stopped-flow spectrometry. (B) “Turn-on” of fluorogenic HELIOS probes 14 and 15 by reaction with TCO. (C) Measured second-order rate constants in PBS at 37 °C for PeptoBrushes 1–3. (D) Measured second-order rate constants per single TCO unit (n = 5, SD < 0.5%). Data for PeptoBrush 1 (corrected) is based on reactive TCO units per polymer as determined by reaction with 17 and UV/vis spectroscopy. (E) Correlation of the reaction rate of PeptoBrush 1 with the lipophilicity (C log D7.4 values) of Tz 14, 15, 18, and 19. C log D7.4 values were calculated using the software Chemicalize. For Tz 19, M = metal ion.