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. 2020 Mar 3;9:e52539. doi: 10.7554/eLife.52539

Figure 1. SLAMF6 is constitutively expressed on T cells and increases upon activation.

Figure 1.

(A–C) SLAMF6 expression in human TIL412 cells, activated for five days. (A) Flow cytometry at the indicated time points. (B) Median fluorescence intensity (MFI) of SLAMF6, days 1–5. (C) Quantitative RT-PCR for SLAMF6. RNA was extracted at the indicated time points. Data normalized to HPRT expression at each time point and to the basal expression level on day 0. One-way ANOVA. **, p<0.01, ***, p<0.001. (D) SLAMF6 expression by flow cytometry in human TIL412 cells activated for 5 days with anti-CD3 or with anti-CD3 plus IL-2, at the indicated time points. (E) SLAMF6 expression by flow cytometry in Pmel-1 mouse splenocytes activated for 6 days, at the indicated time points. (F) Row normalized expression of immune-related genes from RNAseq, clustered according to similar expression patterns. CD4+ T cells from two donors were stimulated with anti-CD3 plus anti-CD28 for 72 hr, RNA was extracted and sequenced. Numbers in the top panel indicate hours. (G) Magnification of cluster C. SLAMF6 is marked.

Figure 1—source data 1. RNA sequencing of healthy donors CD4 T cells along activation.