Figure 5. The contribution of cis and trans SLAMF6 interactions to CD8+ T cell function.
(A) Separated CD8+ splenocytes from Pmel-1 and Pmel-1 x SLAMF6 -/- were co-cultured (1 × 105) overnight at the indicated ratios with non-T splenocytes from both mice splenocytes. IFN-γ secretion was measured by ELISA. (B) SLAMF6 expression on EL4 parental or transfected (SLAMF6 or empty) cells. (C) Pmel-1 or Pmel-1 x SLAMF6 -/- splenocytes were activated for 7 days with gp10025-33 peptide and IL-2 (30 IU/ml) and then incubated overnight with gp10025-33 pulsed EL4 cells (empty or SLAMF6 transfected), at a 1:1 effector-to-target ratio. IFN-γ secretion was measured by ELISA. IFN-γ values are normalized to the results of EL4-empty for each mouse splenocytes. One-way ANOVA test. *, p<0.05, **, p<0.01, ***, p<0.001.