Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background Mus musculus Female) | C57BL/6 | Harlan laboratories | C57BL/6 | |
| Strain, strain background Mus musculus Female) | Pmel-1 | This paper | Pmel-1 | Kind gift from M. Baniyash |
| Strain, strain background Mus musculus Female) | SLAMF6 -/- | This paper | SLAMF6 -/- | Kind gift from I. Shachar |
| Strain, strain background Mus musculus Female) | Pmel-1 x SLAMF6 -/- | This paper | Pmel-1 and SLAMF6-/- mice were bred to generate Pmel-1 X SLAMF6-/- mice according to the ethics requirements (Authority for biological and biomedical models, Hebrew University, Jerusalem, Israel). | |
| Genetic reagent Mus musculus | SLAMF6 | SINO biological | HG11945-UT | pCMV3-mSLAMF6 |
| Cell line Mus musculus | B16-F10/mhgp100 | This paper | Kind gift from Ken-ichi Hanada, Surgery Branch, NCI, NIH | |
| Cell line Mus musculus | EL4 | This paper | Kind gift from Lea Eisenbach, Weizmann Institute, Israel | |
| Cell line (Homo sapiens) | TIL412 | This paper | The cells were maintained in Lotem’s laboratory | |
| Biological sample (Homo sapiens) | PBMCs | This paper | Blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium | |
| Antibody | Monoclonal Rat anti mouse CD16/32 (93) | Biolegend, San Diego, CA | 101302 | 0.2 μg/100 μl |
| Antibody | Monoclonal mouse anti mouse SLAMF6 (330-AJ) | Biolegend, San Diego, CA | 134610 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse TNFα (MP6-XT22) | Biolegend, San Diego, CA | 506314 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD19 (6D5) | Biolegend, San Diego, CA | 115521 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD44 (IM7) | Biolegend, San Diego, CA | 103016 | 0.2 μg/100 μl |
| Antibody | Monoclonal mouse anti mouse TIM3 (RMT3-23) | Biolegend, San Diego, CA | 119706 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse LAG3 (C9B7W) | Biolegend, San Diego, CA | 125210 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD3 (145–2 C11) | Biolegend, San Diego, CA | 100302 | 1 μg/ml |
| Antibody | Monoclonal mouse anti human CD3 (UCHT1) | BD Biosciences, San Jose, CA | 550368 | |
| Antibody | Monoclonal mouse anti human CD28 (CD28.2) | BD Biosciences, San Jose, CA | 556620 | |
| Antibody | Monoclonal rat anti mouse IFNγ (XMG1.2) | Biogems, Westlake Village, CA | 80812 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD8 (53–6.7) | Biogems, Westlake Village, CA | 10122 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse GZMB (NGZN) | Biogems, Westlake Village, CA | 72212 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD4 (GK1.5) | Biogems, Westlake Village, CA | 06112 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD25 (PC61.5) | Biogems, Westlake Village, CA | 07312 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD62L (MEL-14) | eBioscience, CA | 25-0621-81 | 0.2 μg/100 μl |
| Antibody | Monoclonal mouse anti mouse Vb13 (MR12-3) | eBioscience, CA | 17-5797-82 | 0.2 μg/100 μl |
| Antibody | Monoclonal Armenian hamster anti mouse CD69 (H1.2F3) | eBioscience, CA | 14-0691-82 | 0.2 μg/100 μl |
| Antibody | Monoclonal Armenian hamster anti mouse CD279 (J43) | eBioscience, CA | 12-9985-82 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse CD244 (eBio244F4) | eBioscience, CA | 14-2441-82 | 0.2 μg/100 μl |
| Antibody | Monoclonal Syrian hamster anti mouse CD137 (17B5) | eBioscience, CA | 12-1371-82 | 0.2 μg/100 μl |
| Antibody | Monoclonal anti human SLAMF6 (REA339) | Miltenyi Biotec, Bergisch Gladbach, Germany | Cd352 | 0.2 μg/100 μl |
| Antibody | Monoclonal rabbit anti pS6 (D57.2.2E) | Cell Signaling Technology, Danvers, MA | 4858 | 0.2 μg/100 μl |
| Antibody | Monoclonal rat anti mouse LAG-3 (C9B7W) | InVivoMab, BioXcell, NH | BE0174 | 10 μg/1 ml |
| Antibody | Monoclonal rat anti-Ly108 (3E11) | Merck, Kenilworth, NJ | MABF919 | 1:1000 |
| Antibody | Monoclonal rat anti-SAP (1A9) | Biolegend, San Diego, CA | 690702 | 1:1000 |
| Antibody | Monoclonal mouse anti b-actin (sc-47778) | Santa Cruz Biotechnology, TX | C4 | 1:1000 |
| Antibody | Monoclonal rabbit anti-SHP1 | This paper | 1:1000 Generated in Andre’ Veillette laboratory |
|
| Antibody | Monoclonal rabbit anti mouse CD4 (ab183685) | Abcam | EPR19514 | For immunohistochemistry |
| Antibody | Monoclonal rabbit anti mouse CD8+ (ab203035) | Abcam | ab203035 | For immunohistochemistry |
| Sequence-based reagent | Primers | All primers are listed in the primers table in the Materials and methods section | ||
| Peptide, recombinant protein | MART-126–35 | Biomer Technology, Cheshire, UK | ||
| Peptide, recombinant protein | gp10025-33 | Genscript biotech, NJ | ||
| Commercial assay or kit | IFN-γ ELISA | Biolegend | 430801 | |
| Commercial assay or kit | GM-CSF ELISA | Biolegend | 432201 | |
| Commercial assay or kit | GenElute Mammalian Total RNA kit | Sigma Aldrich, MA | RTN70 | RNA production |
| Commercial assay or kit | qScript cDNA Synthesis kit | Quantabio, Beverly, MA | 95047 | RNA transformed to cDNA |
| Commercial assay or kit | RNeasy 96 kit | Qiagen, Hilden, Germany | 74181 | RNA production |
| Commercial assay or kit | Annexin V apoptosis detection kit | eBioscience | 88-8007-74 | Survival assay |
| Commercial assay or kit | Mouse CD8 T cell isolation kit | Stemcell technologies, Vancouver, CA | EasySep 19853A | CD8 isolation from total splenocytes |
| Commercial assay or kit | Quantibody mouse cytokine array | RayBiotech, Peachtree Corners, GA | QAM-CYT-1 | |
| Chemical compound, drug | IL-2 | Chiron, CA | recombinant human IL-2 | |
| Software, algorithm | FCS express five flow research edition | De Novo software | ||
| Software, algorithm | CellSens Entry 1.8 | Olympus Life Science | Acquisition software for immunohistochemistry | |
| Other | lyse/fix buffer | BD Biosciences | Cat: 558049 | |
| Other | PermII buffer | BD Biosciences | Cat: 558052 |