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. 2020 Mar 4;9:e50434. doi: 10.7554/eLife.50434

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© 2020, Gigante et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — Arl13b^hnn MEFs transfected with ARL13B-RAB23^S23N, ARL13B-GFP, or control plasmid. (A) The ARL13BRAB23 ^S23N fusion protein is detectable in the cilium (acetylated a-tubulin, magenta) by ARL13B antibody (cyan, NeuroMab). (B) Wildtype ARL13B-GFP is present in the cilium in high- (left column) and low- (right column) expressing cells. (C) No ARL13B positive cilia were detected in control plasmid transfected cells. (D) Secondary only controls.