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. 2020 Mar 4;9:e50434. doi: 10.7554/eLife.50434

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Gene (M. musculus) Arl13b MGI Cat# 6115585, RRID:MGI:6115585
Genetic reagent (M. musculus) hennin PMID:17488627 MGI Cat# 3580673, RRID:MGI:3580673 Arl13b null allele
Genetic reagent (M. musculus) em1Tc (V358A) This paper MGI:
6256969
New CRISPR Point mutant
Genetic reagent (M. musculus) FBV/NJ Jackson Laboratory Stock #001800
MGI:2163709
Cell lines (M. musculus) Fibroblast (normal, embryonic) This paper Maintained in Caspary lab
Antibody Anti-Shh (Mouse Monoclonal) Developmental Studies Hybridoma Bank DSHB Cat# 5E1, RRID:AB_528466 1:5
Antibody Anti-Nkx2.2 (Mouse Monoclonal) Developmental Studies Hybridoma Bank DSHB Cat# 74.5A5, RRID:AB_531794 1:5
Antibody Anti-Hb9 (Mouse Monoclonal) Developmental Studies Hybridoma Bank DSHB Cat# 81.5C10, RRID:AB_2145209 1:5
Antibody Anti-Nkx6.1 (Mouse Monoclonal) Developmental Studies Hybridoma Bank DSHB Cat# F55A10, RRID:AB_532378 1:50
Antibody Anti-acetylated a-tubulin (Mouse Monoclonal) Millipore Sigma Sigma-Aldrich Cat# T6793, RRID:AB_477585 1:2500
Antibody Anti-Olig2 (Rabbit Polyclonal) Millipore Sigma Millipore Cat# AB9610, RRID:AB_570666 1:300
Antibody Anti-Arl13b (Mouse Monoclonal) NeuroMab UC Davis/NIH NeuroMab Facility Cat# 73–287, RRID:AB_11000053 1:1000
Antibody Anti-Arl13b (Rabbit Polyclonal Protein Tech Proteintech Cat# 17711–1-AP, RRID:AB_2060867 1:1000
Antibody Anti-Arl13b (Rabbit Polyclonal PMID:17488627 503 1:1000
Antibody Anti-Arl13b (Rabbit Polyclonal PMID:17488627 504 1:1000
Antibody Anti-Arl13b (Rabbit Polyclonal PMID:17488627 505 1:1000
Antibody Anti-Smo (Rabbit Polyclonal) K. Anderson 1:1000
Antibody Anti-IFT88 (Rabbit Polyclonal) B. Yoder 1:1000
Antibody Anti-Arl3 (Rabbit Polyclonal) PMID:8034651 1:1000
Antibody Anti-Inpp5e (Rabbit Polyclonal) Protein Tech Proteintech Cat# 17797–1-AP, RRID:AB_2167120 1:150
Antibody Anti-Gli2 (Guinea Pig Polyclonal) J. Eggenschwiler 1:200
Antibody Anti-Gli3 (Goat Polyclonal) R and D R and D Systems Cat# AF3690, RRID:AB_2232499 1:200
Antibody Anti-Ptch1 (Rabbit Polyclonal) R. Rohatgi 1:150
Antibody Anti-Sufu (Goat Polyclonal) Santa Cruz Santa Cruz Biotechnology Cat# sc-10933, RRID:AB_671172 1:100
Antibody Alexa Fluor goat anti-mouse IgG2a 488 ThermoFisher Thermo Fisher Scientific Cat# A-21131, RRID:AB_2535771 1:300
Antibody Alexa Fluor goat anti-mouse IgG1 488 ThermoFisher Thermo Fisher Scientific Cat# A-21121, RRID:AB_2535764 1:300
Antibody Alexa Fluor goat anti-mouse Ig 488 ThermoFisher Molecular Probes Cat# A-11029, RRID:AB_138404 1:300
Antibody Alexa Fluor goat anti-mouse IgG 568 ThermoFisher Thermo Fisher Scientific Cat# A-11031, RRID:AB_144696 1:300
Antibody Alexa Fluor donkey anti-rabbit IgG 488 ThermoFisher Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792 1:300
Antibody Alexa Fluor donkey anti-rabbit IgG 555 ThermoFisher Thermo Fisher Scientific Cat# A-31572, RRID:AB_162543 1:300
Antibody Alexa Fluor goat anti-rabbit IgG 568 ThermoFisher Thermo Fisher Scientific Cat# A-11011, RRID:AB_143157 1:300
Antibody Alexa Fluor goat anti-mouse IgG2b 568 ThermoFisher Thermo Fisher Scientific Cat# A-21147, RRID:AB_2535783 1:300
Antibody Hoechst nuclear stain Millipore Sigma 94403 1:3000
Sequence-based reagent CRISPR gRNA Millpore Sigma, this paper CCAGTCAATACAGACGAGTCTA
Sequence-based reagent CRISPR donor oligo Millpore Sigma, this paper CCTATATTCTTCTAGAAAACAGTAAGAAGAAAACCAAGAAACTACGAATGAAAAGGAGTCATCGGGCAGAACCAGTGAATACAGACGAGTCTACTCCAAAGAGTCCCACGCCTCCCCAAC
Sequence-based reagent F-231-Cac8I This Paper PCR Primer AAGAATGAAAAGGAGTCAGCG
Sequence-based reagent REV-1 This Paper PCR PrimerTGAACCGCTAATGGGAAACT
Peptide, recombinant protein Arl13bWT-GST This Paper Purified from cells
Peptide, recombinant protein Arl13bV358A-GST This Paper Purified from cells
Peptide, recombinant protein Arl3 (human) PMID:11303027 Purified from cells
Peptide, recombinant protein Cas9 Millipore Sigma C120010 50 μg
Peptide, recombinant protein Cac8I New England Biolabs R0579L Restriction enzyme
Chemical compound, drug Ciliobrevin-D Millipore Sigma 250401 30 μM
Chemical compound, drug [3H]GDP PerkinElmer Life Sciences NET966 3000 cpm/pmol
Chemical compound, drug GTPgS35 PerkinElmer Life Sciences NEG030H
Software, algorithm ImageJ software ImageJ (http://imagej.nih.gov/ij/) ImageJ, RRID:SCR_003070
Software, algorithm GraphPad Prism software GraphPad Prism (https://graphpad.com) GraphPad Prism, RRID:SCR_002798 Version 8.0.0