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. 2020 Mar 10;11:142. doi: 10.3389/fimmu.2020.00142

Figure 3.

Figure 3

Increased expression of interleukin 1 receptor associated kinase 1 (IRAK1) in infiltrating B cells, myeloid dendritic cells (mDCs), and macrophages of hyperplastic myasthenia gravis (MG) thymuses. (A) Double immunofluorescence stainings of normal thymus (upper panels) and hyperplastic MG thymuses from corticosteroid-naïve (MG) and corticosteroid-treated (MG-Cortico) MG patients (middle and lower panels) for the expression of IRAK1 (red) in combination with: CD20 (green), marker for B cells; cytokeratin (CK, green), marker of epithelial cells; CD11c (green), marker for mDCs; and CD68 (green), marker for macrophages. The arrows in the middle panels indicate double-positive cells. Blue staining shows 4′,6-diamidino-2-phenylindole (DAPI)-positive nuclei. The inset in MG IRAK-1/CD20 panel shows cells stained only for IRAK1 present in the main panel (dashed box). The insets in MG IRAK1/CD11c panel show enlargement of mDCs cells expressing IRAK1, present in the main panel. HC, Hassall's corpuscle. Magnification bars: 50 μm in the main panels and in MG and MG-Cortico IRAK1/CD20 insets; 20 μm in the MG IRAK1/CD11c insets. (B) Mean percentage (±SEM) of IRAK1-positive cells estimated on total DAPI-positive cells, total CD20-positive B cells, total CD11c-positive mDCs, and total CD68-positive macrophages in six control thymuses, five corticosteroid-naïve (MG), and four corticosteroid-treated (MG-Cortico) MG patients (four adjacent fields for sample group). p values were assessed by the Kruskal–Wallis test for multiple comparisons followed by the Dunn's post-hoc test, *p < 0.05; **p < 0.01.