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. 2020 Mar 10;11:189. doi: 10.3389/fmicb.2020.00189

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Copyright © 2020 Kapach, Nuri, Schmidt, Danin, Ferrera, Savidor, Gerlach and Shai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the effect of mutations in acrB and ramR. (A) A consensus sequence logo of the switch loop was generated through https://weblogo.berkeley.edu/ using the multiple sequence alignment tool CLUSTAL 2.1. Sequences of 998 Gram-negative bacterial species and strains were used. (B) Quantitative reverse transcriptase-polymerase chain reaction (PCR) analysis of the relative expression of the ramR (left panel) and acrB (right panel) mRNA levels, in mid-log phase bacteria. The values are average of three biological replicates, each performed in triplicates. Statistical analysis was done using t-test. ^∗P < 0.05, ^∗∗P < 0.01.