FIGURE 6.

Mutation in skp improved the growth in sub-minimal inhibitory concentration concentrations of C8-K5L7, whereas double mutant was hypersusceptible to colistin. Growth curves of bacterial strains (A) with C8-K5L7 at 3.125 μM, (B) with colistin at 1.56 μM, or (C) without peptide. (D) Growth rates of the strains at the different peptide concentrations. OD₆₀₀ was monitored every 20 min for 18 h. The values are average of at least three biological replicates, each performed in duplicates. Error bars indicate the standard error. Statistical analysis was performed using Tukey multiple-comparisons test for all the time points. Statistically significant differences are indicated as a≠b≠c≠d, P < 0.05.