TABLE 1.
Bacterial strains used in this study and their MICs.
| Bacterial strain | Relevant characteristics/genotypea,b,c | Source | 4DK5L7 MIC (μM) | seg5D MIC (μM) | Colistin MIC (μM) | C8-K5L7 MIC (μM) |
| WT | S. typhimurium 14028s | ATCC | 6.25–12.5 | 6.25–125 | 3.125 | 6.25 |
| Control strain | skpWT, ramRWT, acrbWT, yeiUWT, rfaYWT | This study | 12.5 | 6.25–12.5 | 1.56–3.125 | 6.25 |
| RL1 | skp, ramR, acrb, yeiU, rfaY | This study | 200 | 50 | 6.25 | 6.25–12.5 |
| acrB mut | acrB | This study | 12.5 | 6.25 | ND | ND |
| Double mutant | acrB, ramR | This study | 25 | 6.25–12.5 | 1.56 | 6.25 |
| ramR mut | ramR | This study | 12.5 | 6.25 | ND | ND |
| skp mut | Skp | This study | 25 | 50 | 3.125 | 6.25 |
| Triple mutant | skp, ramR, acrB | This study | 50 | 50 | 3.125 | 6.25 |
| Control strain HA | acrbWT-HA | This study | 12.5 | ND | ND | ND |
| RL1 HA | skp, ramR, acrb-HA, yeiU, rfaY | This study | >50 | ND | ND | ND |
| acrB mut HA | acrb-HA | This study | 12.5 | ND | ND | ND |
| Double mutant HA | ramR, acrb-HA | This study | 25 | ND | ND | ND |
| ramR mut HA | ramR, acrbWT-HA | This study | 12.5 | ND | ND | ND |
aItalics indicate on a point mutation in the mentioned gene. bskpWT, ramRWT, acrbWT, yeiUWT, rfaYWT indicate on a WT allele. cHA indicates on an HA epitope tag that was attached to the C terminus of the gene. ND indicates not determined.