Fig. 4.

Colony formation escalation in a p53 mutant cell line. a G53 colony formation (crystal violet stain) increases across all cell seeding densities (50 to 600 cells) over 14 days compared to G52 (inset magnification × 20). b Colonies were quantified using a stereomicroscope and colony counting pen. G53 colony counts consistently increase over time with increasing cell density, whereas G52 demonstrates no change in formation