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. 2020 Mar 16;10:4793. doi: 10.1038/s41598-020-61514-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EcR and Usp fused to APEX2 and BioID2 enzymes bind genomic sites of EcR and Usp. (A) Distribution of EcR-BioID2 (EcR-Bio), EcR-APEX2 (EcR-APEX), BioID2 (Bio) and APEX2 (APEX) on EcR-bound sites (which were defined using control Drosophila S2 cell line expressing EcR in relation to the corresponding input DNA) in Drosophila S2 cells after 1-hour treatment with 20E. (B) Distribution of Usp-BioID2 (Usp-Bio), Usp-APEX2 (Usp-APEX), BioID2 (Bio) and APEX2 (APEX) on Usp-bound sites (which were defined using control Drosophila S2 cell line expressing Usp in relation to the corresponding input DNA) in Drosophila S2 cells after 1-hour treatment with 20E. Protein binding level was estimated by ChIP-Seq using anti-FLAG antibodies. Heatmaps represent enrichment of ChIP-Seq signal over Input DNA (presented as the ratio of the number of reads at a given point). Pile-up profiles were calculated as a median of binding levels of corresponding proteins in a given distance out from the binding site.