Figure 4.

Zebrafish injected with tick saliva develop hemorrhagic anaphylactic-type reactions (Experiment 1). (A) Accumulated zebrafish allergy was compared between different groups by a one-way ANOVA test (p < 0.05; N = 7–9 biological replicates). (B) Accumulated zebrafish survival in the different groups was compared by a Cox proportional survival regression test (p < 0.05; N = 7–9 biological replicates). (C) Signs of hemorrhagic anaphylactic-type reactions in zebrafish 29-1 injected with α-Gal + PGE₂. Fish No. 29-1 died on day 1. (D) Representative comparison between necropsied control fish No. 30-1 injected with PBS and fish No. 26-1 injected with tick saliva. Evidence of hemorrhage is shown in organs of a saliva-injected fish.