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. 2020 Mar 16;10:4751. doi: 10.1038/s41598-020-61825-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accelerated ectopic bone formation induced by intermittent administration of PTH. (a) Representative intravital two-photon microscopy images of ectopic bone formation in untreated (upper panels) and PTH-treated (lower panels) Col2.3-ECFP/TRAP-tdTomato mice from day 7 to day 21 after CS implantation. Cyan: OBs expressing Col2.3-ECFP; red: OCs expressing TRAP-tdTomato; blue: CFs/bone (SHG). Scale bar, 100 μm. (b) Micro-CT analysis of ectopic-bone volume, with and without PTH treatment, at 7, 10, 14, and 21 days after CS implantation. n = 6–8/group. (c) Time-dependent changes in CFOI, with and without PTH administration. n = 7–11, representative of images collected from 4–5 mice/group. (d) Eccentricity from day 7 to day 21 after CS implantation. n = 7–11, representative of images collected from 4–5 mice/group. (e) OCI from day 7 to day 21 after CS implantation. n = 7–11, representative of images collected from 4–5 mice/group. Data are presented as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant (Mann-Whitney test).