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. 2020 Mar 10;10:304. doi: 10.3389/fonc.2020.00304

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Copyright © 2020 Sadeghi, Ordway, Rafiei, Borad, Fang, Koomen, Zhang, Yoder, Johnson and Damaghi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acid adapted cells show partial EMT phenotype. (A) q-RT-PCR-analysis and (B) IF of EMT marker at RNA and protein level respectively show both markers of epithelial and mesenchymal phenotype are present in acid adapted cells confirming their transient EMT phenotype. (C) Analysis of RNA sequencing shows a mixed epithelial and mesenchymal markers. Heatmap plot for EMT related deferentially expressed genes in AA-MCF7 compared to MCF7. Each row represents a gene and each columns stands for a sample. Cells color is correlated to gene count in the corresponding sample. Color code for gene count: red, high expression; green, low expression.