FIGURE 7.

Effect of rapamycin on the L-Pro- and L-Gln-mediated improvement in development of mouse embryos cultured in isosmotic (270 mOsm/kg) medium at LD. Zygotes (20–22 h post-hCG) were cultured for 5 days in no amino acids (no a.a.), 400 μM L-Pro alone (A), 1 mM L-Gln alone (B), 10 nM rapamycin alone or 10 nM rapamycin in the presence of (A) 400 μM L-Pro or (B) 1 mM L-Gln. The percentage of zygotes that developed to the designated stages over the 5 days was recorded. D indicates the day of development; embryos were analyzed from at least three independent biological replicates. Bars with different letters are significantly different within each day of development, determined using Fisher’s exact test, with Bonferroni correction for multiple comparisons (P < 0.0125).