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. 2020 Mar 10;11:212. doi: 10.3389/fgene.2020.00212

FIGURE 3.

FIGURE 3

hsa_circ_0097435 promoted apoptosis in cardiomyocytes. (A) AC16 cells were transfected with an siRNA vector targeting hsa_circ_0097435. The expression level of hsa_circ_0097435 was detected by qRT-PCR. SC, scramble control; siRNA, siRNA targeting the junction region in hsa_circ_0097435. ***P < 0.001 versus SC. n = 3. (B) AC16 cells were transfected with an hsa_circ_0097435 siRNA-expression vector and treated with 2 μM DOX for 12 h. Apoptosis was evaluated by performing TUNEL assays. Green, TUNEL-positive nuclei; blue, DAPI-stained nuclei. Scale bars, 100 μm. (C) The apoptosis rate calculated from three independent experiments. ***P < 0.001 versus SC. n = 3. (D) AC16 cells were transfected with an hsa_circ_0097435-overexpression vector. The expression level of hsa_circ_0097435 was detected by qRT-PCR. EV: empty vector. ****P < 0.0001 versus EV. n = 3. (E) AC16 cells were transfected with the hsa_circ_0097435-overexpression vector and treated with 1 μM DOX for 12 h. Apoptosis was detected by performing TUNEL assays. Green, TUNEL-positive nuclei; blue, DAPI-stained nuclei. Scale bars, 100 μm. (F) The rate of apoptosis was calculated using data from three independent experiments. ***P < 0.001 versus EV. n = 3.