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. 2020 Mar 10;11:407. doi: 10.3389/fimmu.2020.00407

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Copyright © 2020 Ngamsri, Jans, Putri, Schindler, Gamper-Tsigaras, Eggstein, Köhler and Konrad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of stromal cell-derived factor-1 (SDF-1) and its receptors CXCR4 and CXCR7 in acute inflammation. (A) SDF-1, CXCR4, and CXCR7 mRNA levels were evaluated in peritoneal, lung and liver tissue 4 h after zymosan (n = 8–12) or (B) fecal administration (n = 6–12). (C) Immunofluorescence detection of the expression of SDF-1, CXCR4, and CXCR7 (all green) in peritoneal tissue (Cyto; cytokeratin; red) 4 h after zymosan (63x original magnification). Images are representatives of n = 3 experiments. Intensity was measured by ImageJ. (D) The release of SDF-1α in the peritoneal cavity, in the plasma, and in the bone marrow of wild type mice was detected by using ELISA (n = 6–12). Student's t-test was used for statistical analysis. Data are presented as box and whisker graph with error bars indicating the range from minimum to maximum value; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.