Skip to main content
. 2020 Mar 10;11:407. doi: 10.3389/fimmu.2020.00407

Figure 2.

Figure 2

CXCR4 and CXCR7 treatment dampens neutrophil migration into inflamed tissue through A2B purinergic signaling. (A) We evaluated the influx of polymorphonuclear leukocytes (PMNs) into the peritoneal lavage, lung and liver tissue in wild type (n = 8–12) and A2B–/– animals (F) (n = 6–12) with or without zymosan administration by flow cytometry. Animals were treated with AMD3100 (CXCR4-antagonist) or CCX771 (CXCR7-antagonist). PMN infiltration was also shown by immunohistochemistry, where PMNs were marked brown and counted per high power field (B,G) (n = 16). Representative histological examination of the (C,H) peritoneum, (D,I) lung and (E,J) liver tissue in wild type and A2B–/– animals are shown. Images are representatives of n = 3 experiments. For statistical analysis, one-way ANOVA was used with Bonferroni post-hoc test. Data are presented as box and whisker graph with error bars indicating the range from minimum to maximum value; *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001.