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. 2020 Mar 10;11:407. doi: 10.3389/fimmu.2020.00407

Figure 5.

Figure 5

Impact of the SDF-1 receptors CXCR4 and CXCR7 on the release of inflammatory cytokines and intracellular pathways. (A) After the induction of peritonitis by zymosan, effects of AMD3100 or CCX771 on the gene expression of TNFα and IL6 were evaluated in peritoneal tissue of wild type and (D) A2B–/– mice. (B) The release of inflammatory cytokines (TNFα and IL6) and chemokines (CXCL1 and CXCL2/3) in the peritoneal cavity of wild type and (E) A2B–/– animals was detected. (C) Effects of CXCR4 and CXCR7 inhibition on the phosphorylation of intracellular ERK1/2 and NF-κB subunit 65 in peritoneal tissue of wild type and (F) A2B–/– animals 4 h after NaCl or zymosan with or without AMD3100- or CCX771 were determined. Blots are representatives of n = 3 experiments. Intensity of the blots was evaluated by ImageJ. For statistical analysis, one-way ANOVA was used with Bonferroni post-hoc test. Data are presented as box and whisker graph with error bars indicating the range from minimum to maximum value; n = 6–12; *p < 0.05; **p < 0.01; ***p < 0.001, and ****p < 0.0001.

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