Figure 7.

Impact of CXCR4 and CXCR7 inhibition on microvascular permeability and cytokine release during polymicrobial peritonitis. (A) Protein extravasation was evaluated in the peritoneal cavity 4 h after fecal solution in wild type animals (n = 8–12). (B) mRNA of tight junction proteins was measured in peritoneal tissue of wild type animals (n = 6–12). (C) Protein levels of TJP1 and occludin were quantified in peritoneal tissue (representative blot of n = 3 experiments) and also (D) gene expression of inflammatory cytokines TNFα and IL6 (n = 6–8). (E) TNFα, IL6, CXCL1, and CXCL2/3 release was determined in the peritoneal cavity at indicated time points and conditions (n = 8). (F) Effects of CXCR4- and CXCR7 blockade on the phosphorylation of ERK1/2 and NF-κB subunit 65 in peritoneal tissue of wild type were identified (representative blots of n = 3 experiments). Intensity of the blots was evaluated by ImageJ. For statistical analysis, one-way ANOVA was used with Bonferroni post-hoc test. Data are presented as box and whisker graph with error bars indicating the range from minimum to maximum value; *p < 0.05; **p < 0.01; ***p < 0.001, and ****p < 0.0001.