FIGURE 2.

The SvrPm3^a1/f1-RNAi transgene is constitutively expressed in transgenic wheat lines and silences three B.g. tritici effectors. (A) si-Fi software predictions of SvrPm3^a1/f1-RNAi transgene-derived siRNAs that are efficient (red lines) or inefficient (blue lines) in directing silencing of the three putative target effectors SvrPm3^a1/f1, Bgt_Bcg-6, and Bgt_Bcg-7. On the y-axis is the number of siRNAs that bind to a given locus on the x-axis. (B) Quantification of transgene-derived ssRNA and dsRNA transcripts from non-infected samples. (C–E) Quantification of target gene transcripts from infected samples. Samples were collected 3 days post infection. Error bars represent the standard error. Statistical difference was tested using ANOVA (B:dsRNA,D,E) or Welch ANOVA (B:ssRNA,C), according to the data distribution and variance. Different letters indicate statistical significance.