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Primer list.
| Gene | Forward primer | Reverse primer |
| Lgr5 | TCTTCACCTCCTACC TGGACCT | GGCGTAGTCTGCTATG TGGTGT |
| Olfm4 | CCAGCTGGAGGTGG AGATAA | GCTGATGTTCACCA CACCAC |
| Wnt3 | CAAGCACAACAATGA AGCAGG | TCGGGACTCACGGTG TTTCTC |
| Axin2 | GAGTAGCGCCGTGTTA GTGACT | CCAGGAAAGTCCGGAAGA GGTATG |
| Fzd5 | CTTGTTTCCAAAGTCCAAT CAAGTG | GCCTACTCTTCACCCTTC TTTAACG |
| Pygo2 | GTTTGGGCTGTCCTGAA AGTCTG | ATAAGGGCGCCGAA AGTTGA |
