Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 10;11:241. doi: 10.3389/fphar.2020.00241

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Chen, Ma, Yang, Li, Lin and Gong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

FGF21 is upregulated in in vivo and in vitro models of renal injury. (A–C) Representative immunoblotting analysis of FGF21 and β-Klotho in HK-2 cells. Protein levels of FGF21 and β-Klotho were quantified by densitometry, with β-actin as protein loading control. (D,E) Immunofluorescence intensity of FGF21 in HK-2 cells (scale, 100 μm; DAPI: blue, FGF21: green). (F–H) Representative immunoblotting analysis of FGF21 and β-Klotho in mouse renal tissue. Expression levels of FGF21 and β-Klotho were quantified by densitometry, with GAPDH as protein loading control. (I) Immunohistochemistry staining of FGF21 in mouse renal tissue (scale bar: 100 μm). (J) Quantitative immunohistochemistry analysis of FGF21. ***P < 0.001, **P < 0.01, *P < 0.05.